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Response surface optimization of glucose production from liquid pineapple waste using immobilized invertase in PVA-alginate-sulfate beads

Mohd. Zain, Nor Azimah and Seker, Darshini Chandera (2014) Response surface optimization of glucose production from liquid pineapple waste using immobilized invertase in PVA-alginate-sulfate beads. Separation and Purification Technology, 133 . pp. 48-54. ISSN 1383-5866

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Official URL: http://dx.doi.org/10.1016/j.seppur.2014.06.018

Abstract

Pineapple waste is known as a byproduct of the pineapple processing industry and typically consists of residual pulp, peels and skin. In this study, the conversion of sucrose to glucose was performed with the help of Baker's yeast invertase. Response Surface Methodology (RSM) comprising Box-Behnken design was successfully applied in this study as a tool to evaluate the interactive effects and to obtain the optimum operating conditions for the enzymatic hydrolysis. Four parameters were varied: pH (4-6), temperature (40-60 °C), agitation rate (100-200 rpm) and amount of immobilized beads (3-6 g). Results revealed that the optimum operating conditions derived via RSM were: pH 4.74, temperature 50.11°C, agitation rate of 147.68 rpm and amount of immobilized beads used were 4.45 g. The experimental yield of glucose was found to be 111.42 g/L (850.5%) and sucrose 25.70 g/L (32.3%) under the optimum conditions, which correlated well with the maximum predicted value of glucose and sucrose 110.13 g/L (840.7%) and 25.93 g/L (32.6%) respectively. Hydrolysis of the liquid pineapple waste showed that 95% of sucrose content in the waste was hydrolyzed to glucose after 3 h of hydrolysis and the overall glucose generated from the hydrolysis was approximately 1131% of its original content.

Item Type:Article
Uncontrolled Keywords:invertase, liquid pineapple waste
Subjects:Q Science > QH Natural history
Divisions:Biosciences and Medical Engineering
ID Code:62460
Deposited By: Widya Wahid
Deposited On:14 Jun 2017 10:03
Last Modified:14 Jun 2017 10:03

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