Growth and metabolism of lactobacillus salivarius and lactobacillus delbruekii bulgaricus isolated from mothers milk in diffrent types of probiotic

Abstract

Due to health promoting effects reported for probiotics, these strains are increasingly incorporated into functional food and pharmaceutical markets. This study investigates the growth and metabolism of probiotic strains isolated from mother's mi I k in different types of prebiotic as a carbon source focusing on the production of short-chain fatty acid (SCFAJ such as lactic acid, butyric acid, propioric acid and succinic acid, Two probiotic bacteria I strains namely Lactobacillus salivarius and Lactobacillus delbreuckii bulgaricus, which were isolated from mother's milk, were evaluated both in pure and mixed cultures. The growth kinetics and metabolite profiling of SCFA were studied in different types of carbon sources such as inulin, FQ5 and Arabic gum where growth in glucose acted as control, The best growth profile was obtained using inulin in mixed cultures with maximum cell mass production of 8.23 g L', followed by FOS and glucose with values of 7.67 g L ' and 6.43 g L ' respectively, whereas specific growth rate for this culture was 0.33 h 1. Furthermore, growth of L salivarius in glucose gave the highest succinic acid production (3.99 g L_l) compared to other types of prebiotics and SCFA. Lbulgaricus produced! highest amount of lactic acid (2.39 g L 1) after 24 h of cultivation when glucose was used as the carbon source. On the other hand, growth of mixed culture of Lbulgaricus and Lsdlivarhs gave high succinic acid production (around 2.62 g L ') in F05 after 24 h cultivation. Mixed cultures of L. bulgaricus andl L salivarius in F05 gave the highest amount of total SCFA (6.47 g L 1}. As a condusion, the coneladon of the response would help to determine tfie choice of a good prebiotics for t h e probiotic's strains in order to enhance the beneficial effect to human health.