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Total phenolic content and antioxidant activity of Quercus infectoria galls using supercritical CO2extraction technique and its comparison with Soxhlet extraction

M. N., Hasmida and Md. Salleh, Liza and A. R., Nur Syukriah and Y., Mohd. Harisun and Che Yunus, Mohd. Azizi and Abdul majid, Fadzilah Adibah (2015) Total phenolic content and antioxidant activity of Quercus infectoria galls using supercritical CO2extraction technique and its comparison with Soxhlet extraction. Pertanika Journal Of Science And Technology, 23 (2). pp. 287-295. ISSN 0128-7680

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Abstract

Quercus infectoria gall, which is known as manjakani in Malaysia, was traditionally used in treating diseases. The bioactive compounds from the galls can be extracted using various extraction methods. In this study, supercritical carbon dioxide (SC-CO2) extraction was used to study the effects of CO2 flow rate on the yield, total phenolic content and antioxidant activity of Q. infectoria extract by fixing the pressure and temperature at the highest density (P: 30 MPa, T: 40°C). The results were compared with those acquired from the Soxhlet extraction method. The results showed that the Soxhlet extraction had a higher percentage of extraction yield than SC-CO2 extraction. The selectivity of Q. infectoria extracts using SC-CO2 extraction was better than the Soxhlet extraction method. Meanwhile, the extraction efficiency using the SC-CO2 extraction ranged from 46% to 53%. The SC-CO2 extraction also yielded higher total phenolic content than using the Soxhlet extraction method when 2 mL/min of CO2 flow rate was applied (203.53 mg GA/g sample). This study also revealed that the extracts from the SC-CO2 extraction showed a better radical scavenging activity compared to the Soxhlet extraction when analysed using DPPH (2,2-diphenyl-1-picryl hydrazyl) radical scavenging activity assays.

Item Type:Article
Uncontrolled Keywords:quercus infectoria, radical scavenging activity, radical scavenging activity, soxhlet extraction, supercritical carbon dioxide extraction
Subjects:A General Works
ID Code:59033
Deposited By: Haliza Zainal
Deposited On:04 Dec 2016 12:07
Last Modified:11 Apr 2017 12:48

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