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Sustainable production of the emulsifier methyl oleate by Candida rugosa lipase nanoconjugates

Che Marzuki, Nur Haziqah and Mahat, Naji A. and Huyop, Fahrul and Aboul Enein, Hassan Y. and Abdul Wahab, Roswanira (2015) Sustainable production of the emulsifier methyl oleate by Candida rugosa lipase nanoconjugates. Food And Bioproducts Processing, 96 . pp. 211-220. ISSN 9601-481

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Official URL: http://dx.doi.org/10.1016/j.fbp.2015.08.005


Acid functionalization of multi-walled carbon nanotubes (F-MWCNTs) using a mixture of HNO3 and H2SO4 (1:3, v:v) was used as support materials for the adsorption of Candida rugosa lipase (CRL) as nanoconjugates (CRL-MWCNTs) for producing methyl oleate. To evaluate the competency of the CRL-MWCNTs nanoconjugates, parameters viz. reaction time, surfactant as well as thermostability and reusability were investigated. The characterization of CRL-MWCNTs nanoconjugates using Fourier transform infrared spectroscopy, Field Scanning Electron Microscopy and Transmission Electron Microscopy revealed successful attachment of CRL onto the F-MWCNTs. Utilization of CRL-MWCNTs nanoconjugates resulted in a higher acid conversion in the synthesis of methyl oleate (79.85% at 11 h of reaction time) when compared with the free CRL i.e. an approximately 1.5-fold improvement over the free CRL. The highest percentage of esterification (83.62%) was observed following the use of nonionic surfactant when compared with the anionic and cationic ones. The CRL-MWCNTs nanoconjugates could be used up to 5 cycles, retaining 50% of its residual activity. Since the preparation of the CRL-MWCNTs nanoconjugates was facile and cheap while producing reasonable yield, the CRL-MWCNTs nanoconjugates developed here were found as promising biocatalysts for the production of methyl oleate.

Item Type:Article
Subjects:A General Works
ID Code:58852
Deposited By: Haliza Zainal
Deposited On:04 Dec 2016 12:07
Last Modified:20 Sep 2017 09:25

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