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Dispersive Micro-Solid Phase Extraction Combined with High-Performance Liquid Chromatography for the Determination of Three Penicillins in Milk Samples

Noorfatimah, Yahaya and Sanagi, Mohd. Marsin (2015) Dispersive Micro-Solid Phase Extraction Combined with High-Performance Liquid Chromatography for the Determination of Three Penicillins in Milk Samples. Food Alytical Methods, 8 (5). pp. 1079-1087. ISSN 1936-6612

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Abstract

A simple, rapid, and environmentally friendly microextraction termed COU-2-D-μ-SPE has been developed for the analysis of three penicillins (i.e., oxacillin, cloxacillin, and dicloxacillin) in milk samples. An ordered mesoporous carbon, COU-2, was synthesized and used as the sorbent in dispersive micro-solid phase for the extraction of selected penicillins prior to high-performance liquid chromatography-ultraviolet detection (HPLC-UV). Several important parameters, namely, pH value, salt addition, desorption solvent, extraction time, desorption time, and amount of COU-2, were investigated and optimized. Under the optimum extraction conditions, the method showed good linearity in the range of 10–5,000 μg L−1 (r2 ≥ 0.9994), low limits of detection (2.0–3.3 μg L−1), acceptable reproducibility (relative standard deviation (RSD) 6.2–8.8 %, n = 9), and satisfactory relative recoveries (80.3–99.5 %) for studied penicillins in milk. The proposed COU-2-D-μ-SPE method has been successfully applied to six commercial milk samples, and the extraction of blank samples indicated that all samples were free from selected penicillin contamination.

Item Type:Article
Subjects:A General Works
ID Code:58319
Deposited By: Haliza Zainal
Deposited On:04 Dec 2016 12:08
Last Modified:15 Sep 2017 17:08

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