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Characterization of aluminum resistant Anoxybacillus sp SK 3-4 isolated from a hot spring

Lim, Jia Chun and Goh, Kian Mau and Shamsir, Mohd. Shahir and Ibrahim, Zaharah and Chong, Chun Shiong (2015) Characterization of aluminum resistant Anoxybacillus sp SK 3-4 isolated from a hot spring. Jourl Of Basic Microbiology, 55 (4). pp. 514-519. ISSN 0233-111X

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Official URL: https://www.ncbi.nlm.nih.gov/pubmed/25523650


The Anoxybacillus sp. SK 3-4, previously isolated from a hot spring, was screened for its heavy metals resistance (Al(3+), Mn(2+), Cu(2+), Co(2+), Zn(2+), and Ni(2+)) and the strain was found to be most resistant to aluminum. Significant growth of the strain was observed when it was grown in medium containing aluminum (200 mg L(-1)-800 mg L(-1)) with relative growth rates ranging between 77% and 100%. A gene encoding the aluminum resistance protein (accession number: WP_021095658.1) was found in genome of strain SK 3-4, which revealed high sequence identity (>95%) to its homologues from Anoxybacillus species. Sequence comparisons with two functionally characterized aluminum resistance proteins, namely G2alt and ALU1-P, showed 97% and 81% of sequence identity, respectively. Four putative metal binding sites were detected in SK 3-4 aluminum resistance protein and G2alt at same amino acid residue positions of 186, 195, 198, and 201. Strain SK 3-4 was found to be able to remove aluminum from aqueous solution. This study demonstrated that Anoxybacillus sp. SK 3-4 could be applied in the treatment of aluminum contaminated wastewater.© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Item Type:Article
Subjects:A General Works
ID Code:58032
Deposited By: Haliza Zainal
Deposited On:04 Dec 2016 12:07
Last Modified:28 Aug 2017 08:29

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