Cloning of beta-xylosidase gene from aspergillus niger

Abstract

Filamentous fungi are known to be efficient producers of xylanolytic enzymes, and most commercial xylanolytic preparations are obtained from fermentations of Aspergillus or Trichoderma species. β-xylosidase genes from Aspergillus niger was amplified using Polymerase Chain Reaction. β-xylosidase gene in Aspergillus niger contains an open reading frame of 2,412 nucleotides which show high similarity to Aspergillus awamori xyl mRNA for β-xylosidase (Accession Number: AB 154359) . The gene encoded 804 amino acid sequence of β-xylosidase. Amino acid analysis shows 99% identities to β-xylosidase from Aspergillus awamori.