Universiti Teknologi Malaysia Institutional Repository

Glucose concentration affects recombinant interferon a-2b production in Escherichia coli using thermo-induction system

Mansey, Mesalhy S. and Ghareeb, Kamel A. and Moghazy, Abdel Naser and Tawfick, Mahmoud M. and Fouda, Mohamed M. and El- Marzugi, Nagib A. and Othman, Nor Zalina and El-Enshasy, Hesham Ali (2014) Glucose concentration affects recombinant interferon a-2b production in Escherichia coli using thermo-induction system. Journal of Applied Pharmaceutical Science, 4 (5). pp. 1-5. ISSN 2231-3354

[img]
Preview
PDF
128kB

Official URL: http://dx.doi.org/10.7324/JAPS.2014.40501

Abstract

In the present work, a recombinant Escherichia coli strain was used for the production of interferon a-2b in both shake flask and in bioreactor. The first part of this research was focused on the investigation of the effect of glucose concentration on the kinetics of cell growth, recombinant protein production and acetate formation. In general, glucose supplementation to culture medium enhanced cell growth when added in concentration between 0-20 g/L. Further increase in glucose level reduces biomass production and enhances acetate accumulation in culture. The results clearly demonstrated that maximal interferon production of 27.7 mg/L was achieved in culture supplemented with 20 g/L glucose. Further improvement in recombinant interferon production process was also achieved by scaling up from shake flask to 16-L stirred tank bioreactor. The maximal volumetric interferon production in bioreactor batch culture was 44.5. mg/L after only 6 hours.

Item Type:Article
Uncontrolled Keywords:batch cultivation, bioreactor cultivation, glucose effect, interferon α-2b, rescharichia coli
Subjects:Q Science > QR Microbiology
Divisions:Others
ID Code:52984
Deposited By: Siti Nor Hashidah Zakaria
Deposited On:01 Feb 2016 11:53
Last Modified:19 Jul 2018 15:22

Repository Staff Only: item control page