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Pilot scale extraction of proteolytic enzyme bromelain from pineapple (ananas comosus)

Loh, Melissa Pei Shian and Syamsul Baharil, Nur Aishah and Ting, Lee Yu and M., Taher and Abdul Majid, Fadzilah Adibah (2005) Pilot scale extraction of proteolytic enzyme bromelain from pineapple (ananas comosus). In: 2nd International Conference on Chemical and Bioprocess Engineering in conjunction with 19th Symposium of Malaysian Chemical Engineers (SOMChE 2005), 8-10 December 2005, Kota Kinabalu, Sabah.



Bromelain is a complex of proteolytic enzymes obtained from pineapple (Ananas comosus) fruits and stem. These proteolytic enzymes are tools that promise an impressive number of medical and therapeutic uses, particularly as anti-inflammatory agents, dietary supplements and analgesics. The purpose of this study was to develop a pilot scale bromelain extraction process. The bromelain extraction method using pineapple fruit core in this study has yielded a total protein content of 9.33 mg/ml with the proteolytic activity of 1400 GDU/ml. An increase in homogenization cycle to increase enzyme yield has proven to be successful. Triple-loop homogenization has shown three times higher proteolytic activity than non-homogenized sample.

Item Type:Conference or Workshop Item (Paper)
Uncontrolled Keywords:Bromelain, homogenized, proteolytic enzyme
Subjects:T Technology > T Technology (General)
Divisions:Chemical and Natural Resources Engineering
ID Code:5270
Deposited By: Norhani Jusoh
Deposited On:18 Mar 2008 10:41
Last Modified:30 Aug 2017 04:30

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