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Bioprocess development for high cell mass and endospore production by Bacillus thuringiensis var israelensis in semi-industrial scale

Elsayed, Elsayed Ahmed and Othman, Nor Zalina and Abd. Malek, Roslinda and Awad, Hassan Mohamed and Aziz, Md. Mijanur and Wu, King and Wadaan, Mohammad Ahmed and El-Enshasy, Hesham Ali (2014) Bioprocess development for high cell mass and endospore production by Bacillus thuringiensis var israelensis in semi-industrial scale. Journal of Pure and Applied Microbiology, 8 (4). pp. 2773-2783. ISSN 0973-7510

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Official URL: https://microbiologyjournal.org

Abstract

The process for high cell mass and spore production by Bacillus thuringiensis var israelensis (Bti) on semi-industrial scale was developed. Cultivations were conducted in shake flask and bioreactor levels to develop a semi-industrial scale process for the production of Bti cells and spores. The composition of the most suitable cultivation medium was further optimized to enhance biomass and spore production and also to reduce the production time. Furthermore, cultivations were performed in 16-L stirred tank bioreactor to investigate the scalability of this process and to study the effect of different aeration rates. The optimization of the main three components of the cultivation medium, i.e. glucose, ammonium sulphate and phosphate, resulted in a significant increase in both cell growth and spore formation by about 32% and 119%, respectively. Scaling up the cultivation process from shake flask to 16-L bioreactor further improved the process. Maximal cell mass of 16.06 g.L-1 concomitant with a spore production of 152x107 spores.mL-1 was achieved in lv.v-1.min-1 aerated culture. In terms of cell growth and spore production, these results were 5- and 8.5-fold higher, respectively, compared with the initial un-optimized medium in shake flask culture. The results obtained in this study improved the process of Bti production in semi-industrial scale through the optimization of both the cultivation medium composition and the aeration rate in pilot scale stirred tank bioreactor.

Item Type:Article
Uncontrolled Keywords:aeration, bacillus thuringiensis var israelensis, batch culture, pilot scale, spore production, stirred tank bioreactor
Subjects:Q Science > QH Natural history
Divisions:Science
ID Code:52018
Deposited By: Siti Nor Hashidah Zakaria
Deposited On:01 Feb 2016 11:53
Last Modified:30 Nov 2018 15:00

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