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Article; food biotechnology enzymatic production of a solvent-free menthyl butyrate via response surface methodology catalyzed by a novel thermostable lipase from Geobacillus Zalihae

Abdul wahab, Roswanira and Basri, Mahiran and Raja Abd. Rahman, Raja Noor Zaliha and Salleh, Abu Bakar and Abdul Rahman, Mohd. Basyaruddin and Chaibakhsh, Naz and Leow, Adam Thean Chor (2014) Article; food biotechnology enzymatic production of a solvent-free menthyl butyrate via response surface methodology catalyzed by a novel thermostable lipase from Geobacillus Zalihae. Biotechnology and Biotechnological Equipment, 28 (6). pp. 1065-1072. ISSN 1310-2818

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Official URL: http://dx.doi.org/10.1080/13102818.2014.978220

Abstract

Most substrate for esterification has the inherent problem of low miscibility which requires addition of solvents into the reaction media. In this contribution, we would like to present an alternative and feasible option for an efficient solvent-free synthesis of menthyl butyrate using a novel thermostable crude T1 lipase. We investigated the effects of incubation time, temperature, enzyme loading and substrate molar ratio and determined the optimum conditions. The high conversion of menthyl butyrate catalyzed by crude T1 lipase in a solvent-free system is greatly affected by temperature and time of the reaction media. The highest yield of menthyl butyrate was 99.3% under optimized conditions of 60 C, incubation time of 13.15 h, 2.53 mg, 0.43% (w/w) enzyme to substrate ratio and at molar ratio of butyric anhydride/menthol 2.7:1. Hence, the investigation revealed that the thermostable crude T1 lipase successfully catalyzed the high-yield production of menthyl butyrate in a solvent-free system. The finding suggests that the crude T1 lipase was a promising alternative to overcome shortcomings associated with solvent-assisted enzymatic reactions

Item Type:Article
Uncontrolled Keywords:central composite rotatable design, esterification, geobacillus zalihae, T1 lipase
Subjects:Q Science
Divisions:Science
ID Code:51922
Deposited By: Siti Nor Hashidah Zakaria
Deposited On:01 Feb 2016 11:53
Last Modified:29 Aug 2018 15:57

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