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Antioxidant and anti-tyrosinase activities from Piper officinarum C.DC (Piperaceae)

Wan Salleh, Wan Mohd. Nuzul Hakimi and Ahmad, Farediah and Yen, Khong Heng (2014) Antioxidant and anti-tyrosinase activities from Piper officinarum C.DC (Piperaceae). Journal of Applied Pharmaceutical Science, 4 (5). pp. 87-91. ISSN 2231-3354


Official URL: http://dx.doi.org/10.7324/JAPS.2014.40516


The present study was carried out to evaluate antioxidant and anti-tyrosinase activities from Piper officinarum stems, as well as investigation of its chemical constituents. In a series of in vitro assays, antioxidant (DPPH radical scavenging and total phenolic content) and anti-tyrosinase (mushroom tyrosinase) activities of various extracts of stem were evaluated. The isolation and purification of the constituents were carried out on the extracts using various chromatographic methods and identified by direct comparison of their spectroscopic data with respective published data. The results showed that the methanol extracts showed the highest DPPH (80.0%) at 1 mg/ml, as well as total phenolic content (50.5%). Phytochemical analysis of the stem extracts have isolated five compounds identified as 4-allyl resorcinol (1), aristolactam AII (2) aristolactam BII (3), stigmast-4-en-3-one (4) and 6-hydroxystigmast-4-en-3-one (5). Compound (1), (2) and (3) showed significant activity towards DPPH radical scavenging (I%=17.3-28.1%), while (2), (3) and (4) demonstrated potent inhibitory effects against tyrosinase mushroom (I%=11.1-24.4%). The results showed that the stem extracts has significant antioxidant activity that may help to discover new chemical classes of natural antioxidant substances

Item Type:Article
Uncontrolled Keywords:anti-tyrosinase, antioxidant, Piper officinarum, Piperaceae
Subjects:Q Science
ID Code:51877
Deposited By: Siti Nor Hashidah Zakaria
Deposited On:01 Feb 2016 11:52
Last Modified:27 Aug 2018 11:41

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