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Sandwiched polymer fibre in fibrin in matrices for the dictation of endothelial cells undergoing angiogenesis

Sukmana, Irza and Djuansjah, J.R.P. (2013) Sandwiched polymer fibre in fibrin in matrices for the dictation of endothelial cells undergoing angiogenesis. In: 2013 International Conference On Science & Engineering In Mathematics, Chemistry And Physics (SCIETECH 2013), 24 January 2013 - 25 January 2013, Jakarta; Indonesia.

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Official URL: http://dx.doi.org/10.1088/1742-6596/423/1/012049


We present here a three-dimensional (3D) sandwich system made by poly(ethylene terephthalate) (PET) fibre and fibrin extracellular matrix (ECM) for endothelial cell dictation and angiogenesis guidance. In this three-dimensional system, Human Umbilical Vein Endothelial cells (HUVECs) were firstly cultured for 2 (two) days to cover the PET fibre before sandwiched in two layer fibrin gel containing HUVECs. After 4 (four) days of culture, cel-to-cel connection, tube-like structure and multi-cellular lumen formation were then assessed and validated. Phase contrast and fluorescence imaging using an inverted microscope were used to determine cell-to-cell and cell-ECM interactions. Laser scanning confocal microscopy and histological techniques were used to confirm the development of tube-like structure and multi-cellular lumen formation. This study shows that polymer fibres sandwiched in fibrin gel can be used to dictate endothelial cells undergoing angiogenesis with potential application in cancer and cardiovascular study and tissue engineering vascularisation.

Item Type:Conference or Workshop Item (Paper)
Uncontrolled Keywords:Fibers, Nanofibers, Polyethylene terephthalates
Subjects:Q Science > QH Natural history
Divisions:Biosciences and Medical Engineering
ID Code:51293
Deposited By: Haliza Zainal
Deposited On:27 Jan 2016 09:53
Last Modified:12 Jun 2017 12:29

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