Universiti Teknologi Malaysia Institutional Repository

Analysis of fluconazole in human urine sample by high performance liquid chromatography method

Hermawan, D. and Md. Ali, N. A. and Wan Ibrahim, Wan Azli and Sanagi, M. M. (2013) Analysis of fluconazole in human urine sample by high performance liquid chromatography method. In: 2013 International Conference On Science & Engineering In Mathematics, Chemistry And Physics (SCIETECH 2013).

Full text not available from this repository.

Official URL: http://iopscience.iop.org/article/10.1088/1742-659...

Abstract

A method for determination of fluconazole, antifungal drug in human urine by using reversed-phased high performance liquid chromatography (RP-HPLC) with ultraviolet (UV) detector was developed. Optimization HPLC conditions were carried out by changing the flow rate and composition of mobile phase. The optimum separation conditions at a flow rate 0.85 mL/min with a composition of mobile phase containing methanol:water (70:30, v/v) with UV detection at a wavelength 254 nm was able to analyze fluconazole within 3 min. The excellent linearity was obtained in the range of concentration 1 to 10 µg/mL with r2 = 0.998. The limit of detection (LOD) and limit of quantitation (LOQ) were 0.39 µg/mL and 1.28 µg/mL, respectively. Solid phase extraction (SPE) method using octadecylsilane (C18) as a sorbent was used to clean-up and pre-concentrated of the urine sample prior to HPLC analysis. The average recoveries of fluconazole in spiked urine sample was 2.4% with RSD of 3.21% (n=3).

Item Type:Conference or Workshop Item (Paper)
Additional Information:Journal of Physics: Conference Series 423 (2013) 012009
Uncontrolled Keywords:antifungal drug, fluconazole, human urine, RP-HPLC, SPE
Subjects:Q Science
Divisions:Science
ID Code:50896
Deposited By: Haliza Zainal
Deposited On:27 Jan 2016 09:53
Last Modified:14 Sep 2017 15:54

Repository Staff Only: item control page