Hermawan, D. and Md. Ali, N. A. and Wan Ibrahim, Wan Azli and Sanagi, M. M. (2013) Analysis of fluconazole in human urine sample by high performance liquid chromatography method. In: 2013 International Conference On Science & Engineering In Mathematics, Chemistry And Physics (SCIETECH 2013).
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Official URL: http://iopscience.iop.org/article/10.1088/1742-659...
Abstract
A method for determination of fluconazole, antifungal drug in human urine by using reversed-phased high performance liquid chromatography (RP-HPLC) with ultraviolet (UV) detector was developed. Optimization HPLC conditions were carried out by changing the flow rate and composition of mobile phase. The optimum separation conditions at a flow rate 0.85 mL/min with a composition of mobile phase containing methanol:water (70:30, v/v) with UV detection at a wavelength 254 nm was able to analyze fluconazole within 3 min. The excellent linearity was obtained in the range of concentration 1 to 10 µg/mL with r2 = 0.998. The limit of detection (LOD) and limit of quantitation (LOQ) were 0.39 µg/mL and 1.28 µg/mL, respectively. Solid phase extraction (SPE) method using octadecylsilane (C18) as a sorbent was used to clean-up and pre-concentrated of the urine sample prior to HPLC analysis. The average recoveries of fluconazole in spiked urine sample was 2.4% with RSD of 3.21% (n=3).
Item Type: | Conference or Workshop Item (Paper) |
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Additional Information: | Journal of Physics: Conference Series 423 (2013) 012009 |
Uncontrolled Keywords: | antifungal drug, fluconazole, human urine, RP-HPLC, SPE |
Subjects: | Q Science |
Divisions: | Science |
ID Code: | 50896 |
Deposited By: | Haliza Zainal |
Deposited On: | 27 Jan 2016 01:53 |
Last Modified: | 14 Sep 2017 07:54 |
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