Md. Noh, Abidah and Mohamad, Siti Hajar and Md. Jahim, Jamaliah and Abdul Murad, Abdul Munir and Abu Bakar, Farah Diba and Muhammad, Nor and Mahadi, Mahadi and Md. Illias, Rosli (2012) Preliminary study of cellobiohydrolase production from aspergillus niger using static liquid culture fermentation. Australian Journal Of Basic And Applied Sciences, 6 (1). pp. 43-49. ISSN 1991-8178
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Official URL: http://www.ajbasweb.com/old/ajbas/2012/January/43-...
Abstract
This study was aimed to investigate the production of recombinant cellobiohydrolase (CBH) in static liquid culture (SLC) fermentation using newly developed recombinant of A. niger. Different environmental parameters and culture conditions were used to attain good cultivation of A. niger in SLC fermentation and highest level of CBH production. The fermentation results were evaluated from CBH production, total biomass, total protein and the residual main carbon source, maltose after 9 days of cultivation. The CBH production was found effectively enhanced under optimized cultivation temperature, initial pH, inoculums size and pre-shaked inoculums. Maximum biomass, protein concentration and enzyme activity were determined as 1.5 g dry weight/ml medium, 3.5 mg/ml and 40 U/ml respectively. The results also show that SLC could enhance production of the recombinant CBH.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Aspergillus niger, cellobiohydrolase production, pre-shaked inoculums |
| Subjects: | Q Science |
| Divisions: | Biosciences and Bioengineering |
| ID Code: | 47403 |
| Deposited By: | Narimah Nawil |
| Deposited On: | 22 Jun 2015 05:56 |
| Last Modified: | 25 Apr 2019 01:18 |
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