Determination of epoxidixed soybean oil in foods stored in glass jars with metal lids using gas chromatography-mass spectrometry

Abstract

A method has been developed for the determination of epoxidized soybean oil (ESBO) in oily foods stored in glass jars with metal lids using gas chromatography-mass spectrometry (GC-MS). ESBO and its internal standard (cis, cis-11,12; 14,15-diepoxyeicosanoate) were isolated from the matrix by transesterification process. The developed method showed good linear dynamic range between 0.7-20 mu g mL(-1) with coefficients of determination (R-2) > 0.9968 and acceptable limit of detection and limit of quantitation of 7 and 23 mg kg(-1), respectively, based on linearity calculations. Analyte recoveries were 90.84 +/- 27.24% for low concentration, 78.05 +/- 11.59% for medium concentration and 99.23 +/- 10.20% for high concentration. This first fully validated GC-MS method was successfully applied for the determination of ESBO in foods stored in glass jar with metal lid. Among the 31 food samples studied, 6 samples were found to exceed the specific migration limit of 60 mg kg(-1) (based on EU Directive 2002/72/EC). The developed method is thus potentially useful for routine analysis for the determination of ESBO.