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Interference elimition of an amperometric glucose biosensor using poly (hydroxyethyl methacrylate) membrane

Abd. Aziz, Azila and Wong, Fui Ling (2011) Interference elimition of an amperometric glucose biosensor using poly (hydroxyethyl methacrylate) membrane. Engineering in Life Sciences, 11 (1). pp. 20-25. ISSN 1618-0240

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A permselective membrane fabricated from photo-cross-linked poly(hydroxyethyl methacrylate) (pHEMA) was studied as a potential selective membrane that can eliminate electrochemical interferences commonly faced by a hydrogen peroxide-based biosensor. The quantitative selection of the permselective membrane was based on the permeabilities of hydrogen peroxide and acetaminophen (AC). AC was used as a model of the interfering substance due to its neutral nature. pHEMA membrane with the cross-linking ratio of 0.043 was found to achieve a selectivity of hydrogen peroxide over AC of 10, while maintaining an acceptable degree of hydrogen peroxide response. A two-layer glucose biosensor model consisting of glucose oxidase entrapped within a freeze-thawed poly(vinyl alcohol) matrix and the cross-linked pHEMA membrane was challenged with AC, ascorbic acid and uric acid. 0.2?mM AC and 0.2?mM ascorbic acid were completely eliminated. However, 0.2?mM uric acid could not be completely eliminated and still gave a bias of approximately 6.6% relative to 5?mM glucose. The results showed that cross-linked pHEMA was quite promising as an interference eliminating inner membrane.

Item Type:Article
Uncontrolled Keywords:glucose biosensor
Subjects:H Social Sciences > HD Industries. Land use. Labor
Divisions:Chemical Engineering
ID Code:45001
Deposited By: Haliza Zainal
Deposited On:27 Apr 2015 04:49
Last Modified:20 Sep 2017 07:18

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