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Characterization of a labrys sp. strain wy1 able to utilize 2,2-dichloropropiote (2,2-DCP) as sole source of carbon

Wen, Yong Wong and Huyop, Fahrul (2011) Characterization of a labrys sp. strain wy1 able to utilize 2,2-dichloropropiote (2,2-DCP) as sole source of carbon. African Journal of Microbiology Research, 5 (20). pp. 3282-3288. ISSN 1996-0808

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Abstract

Industrial chemicals and pesticides from agricultural activities cause a considerable threat to the environment. 2,2-dichloropropionate (2,2-DCP) is a synthetic halogenated compound used as herbicide. High concentration of 2,2-DCP is toxic if released to the environment and may pollute the soil and ground water source. Using current enrichment technique it was expected to isolate a new bacteria species able to degrade a-halocarboxylic acid. Strain Wy1 isolated from soil in Melaka rubber estate was able to utilize 30 mM 2, 2-DCP as the sole source of carbon and energy with maximum chloride ion released of 0.27 mmol/L in the liquid growth medium. The biochemical test and 16S rRNA analysis suggested that the bacterial identity was from the genus Labrys sp., and therefore it was designated as Labrys sp. strain Wy1. The cells doubling time in 30 mM 2,2-DCP liquid minimal medium was 33.44 h. Cell growth was inhibited when grown in liquid minimal medium above 30 mM 2,2-DCP due to the toxicity of 2,2-DCP to the cells. This is the first reported case that the genus Labrys sp. is able to degrade 2,2-DCP.

Item Type:Article
Uncontrolled Keywords:carbon
Subjects:T Technology > TP Chemical technology
Divisions:Built Environment
ID Code:44781
Deposited By: Haliza Zainal
Deposited On:21 Apr 2015 11:31
Last Modified:13 Sep 2017 11:45

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