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Phytochemicals and bioactivity of Piper porphyrophyllum N. E. Br. and Piper sarmentosum Roxb.

Sidek, Raihan Murni (2013) Phytochemicals and bioactivity of Piper porphyrophyllum N. E. Br. and Piper sarmentosum Roxb. Masters thesis, Universiti Teknologi Malaysia, Faculty of Science.

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Abstract

Chemical investigations of Piper porphyrophyllum N. E. Br. and Piper sarmentosum Roxb. have been carried out. The stems of P. porphyrophyllum and the whole plant of P. sarmentosum, each were extracted by maceration using petroleum ether, dichloromethane, ethyl acetate and methanol consecutively. Purification of the crude extracts was carried out using several chromatographic techniques. The structures of the pure compounds were elucidated by spectroscopic methods such as IR, 1D (1H, 13C, DEPT) and 2D (COSY, HMQC, HMBC) NMR and GC-MS. Four compounds have been identified from the petroleum ether extract of P. Porphyrophyllum and were characterized as ß-sitosterol, sesamin, N-isobutyl-15- (3’,4’-methylenedioxyphenyl)-2E,4E,12E-pentadecatrienamide and a new compound, 5-hydroxy-7,2’,4’,6’-tetramethoxyflavone. Meanwhile, 5,7,3’,4’,5’- pentamethoxyflavone was identified from the dichloromethane extract. Four compounds were isolated from P. sarmentosum. The petroleum ether extract afforded 3’,4’-methylenedioxyphenyl-13-tetradecene while the dichloromethane extract gave asaricin and 2-(methyl-2’-propenoate)-4,5- methylenedioxybenzaldehyde. Purification of the methanol extract gave 4-(2’- octenyl)phenol. Pure compounds which were isolated abundantly were screened for cytotoxic study using methylthiazol tetrazolium (MTT) cell proliferation assay. 4- (2’-Octenyl)phenol was found to have the highest cytotoxic effect towards the cell with the percentage of cell inhibition of 12.96%.

Item Type:Thesis (Masters)
Additional Information:Thesis (Sarjana Sains (Kimia)) - Universiti Teknologi Malaysia, 2013; Supervisor : Assoc. Prof. Dr. Farediah Ahmad
Subjects:Q Science > QK Botany
Divisions:Science
ID Code:42194
Deposited By: Haliza Zainal
Deposited On:09 Oct 2014 17:21
Last Modified:17 Aug 2020 10:26

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