Universiti Teknologi Malaysia Institutional Repository

Heat sensitivity between human normal liver (WRL68)l(MCF7) cell lines

Hamdan, Salehhuddin and Elengoe, Asita (2013) Heat sensitivity between human normal liver (WRL68)l(MCF7) cell lines. Journal of Biotechnology Letters, 4 (1). pp. 45-50. ISSN 0976-7045

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Abstract

Hyperthermia is a potential tool for various types of cancer treatments in which body tissue is exposed to high temperature with usually minimal injury or no injury to normal tissues. In the present study, it was aimed to investigate temperature and duration of heat shock on viability of human normal liver (WRL-68) and breast carcinoma (MDA-MB 231) cell lines using MTT and methylene blue assay. These cells were exposed to heat at three different temperatures (38, 40 and 42°C) for five different duration of heat exposure (0.5, 1, 2, 3 and 4 hours). Viability of WRL-68 and MDA-MB 231 cell lines is dependent on temperature and duration of hyperthermic exposure. After increasing temperature from 38 to 42°C, more cancer cells were killed during the same period of heat exposure compare to normal cells. This was due to MDA-MB 231 cells do not have defense system against heat, as do WRL-68 cells. There was a great variation of thermo sensitivity among normal cell and cancer cell. It was observed that WRL-68 cells were killed at 42°C for 3 hours meanwhile MDA-MB 231 cells need temperature of 38°C for 0.5 hours.

Item Type:Article
Uncontrolled Keywords:cell viability, hyperthermia, methylene blue assay
Subjects:Q Science
Divisions:Biosciences and Medical Engineering
ID Code:40275
Deposited By: Narimah Nawil
Deposited On:19 Aug 2014 11:38
Last Modified:17 Mar 2019 12:21

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