Integrated dual nanoprobe-microfluidic system for single cell penetration

Abstract

This paper presents the results in single cell intracellular penetration using the integrated dual nanoprobe-microfluidic system. Current technique for single cell penetration is using a manual controlled nanomanipulator which been attached with a nanoprobe. Due to this, successful single cell penetration depends on the operator skills. We proposed an integrated dual nanoprobe-microfluidic system. The system was being developed to reduce the requirement for skilled operator in single cell electrical measurement. We tested the system function for single cell penetration via finite element simulation. Based on the results, the single cell can be penetrated at fluid velocity of 5.3 μm/s. Currently, the developed system is suitable for cell viability detection application. However, this system has the potential to be used in single cell thermal measurement, single cell drug delivery, and early disease diagnosis.