Universiti Teknologi Malaysia Institutional Repository

Targeted biolistics for improved transformation of impatiens balsamina

Wetten, Andy C. and Thomas, Jean Luc and Wagiran, Alina and Chiurugwi, Tinashe (2012) Targeted biolistics for improved transformation of impatiens balsamina. Methods in Molecular Biology, 847 . pp. 255-265. ISSN 1064-3745

Full text not available from this repository.

Official URL: http://dx.doi.org/10.1007/978-1-61779-558-9_22


A transgenesis programme has been developed for Impatiens balsamina that will allow elucidation of the roles played by individual genes in the flower reversion phenomenon shown by this model species. The lack of explants exhibiting adventitious shooting in I. balsamina hinders Agrobacterium-based transformation, but the multiple shoots that arise from cotyledonary nodes present a suitable target for biolistics. These tissues can be disrupted by the helium blast effect associated with conventional biolistic devices, so we have utilised modifications to the PDS 1000/He equipment originally developed for transformation of fragile insect tissues. By loading microcarriers on to a rigid, rather than flexible, macrocarrier, the blast effect is largely eliminated, and the use of a focussing nozzle allows the bombardment to be concentrated on the target tissues. This approach reduces waste of plasmid DNA and gold microcarriers and achieves transfection at lower, less disruptive helium pressures than would otherwise be necessary to efficiently penetrate below the shoot epidermis and generate heritable transgenic lines.

Item Type:Article
Additional Information:Indexed by Scopus
Uncontrolled Keywords:biolistics, cotyledonary nodes, flower reversion, impatiens balsamina, transformation
Subjects:R Medicine > R Medicine (General)
Divisions:Biosciences and Bioengineering
ID Code:36038
Deposited By: Fazli Masari
Deposited On:02 Dec 2013 04:42
Last Modified:16 Feb 2017 00:29

Repository Staff Only: item control page