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Molecular identification of Malaysian pineapple cultivar based on Internal Transcribed Spacer (ITS) region

Hidayat, Topik and Abdullah, Farah Izana and Kuppusamy, Chandrika and Abdul Samad, Azman and Wagiran, Alina (2012) Molecular identification of Malaysian pineapple cultivar based on Internal Transcribed Spacer (ITS) region. In: 2012 2nd International Conference on Asia Agricultutre and Animal (ICAAA 2012).

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Abstract

Identification of pineapple cultivar solely based upon traditional method which only based on morphology. However, this approach could results in inaccurate cultivar identification and inconsistent. In this study, we conducted phenetic analysis of 9 Malaysian pineapple cultivars based on DNA sequences of the internal transcribed spacer (ITS) region to evaluate utility of the region as a barcode in identification of pineapple cultivars and to determine phenetic relationships among cultivar. Genomic DNA was directly extracted, and ITS region was amplified and sequenced. Phenetic analysis revealed that the pineapple cultivars used in this study were classified into three groups with high sequence similarity among them. This clearly showed that the DNA barcode from ITS region have good discrimination power to distinguish the pineapple cultivar. Since the tree distinctly separated into three group of cultivar, consensus sequences served as DNA barcode for Malaysian pineapple cultivar can be constructed.

Item Type:Conference or Workshop Item (Paper)
Divisions:Biosciences and Medical Engineering
ID Code:34141
Deposited By: Liza Porijo
Deposited On:20 Aug 2017 11:54
Last Modified:06 Sep 2017 12:04

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