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Successful plant regeneration of Orthosiphon stamineus from petiole

Mohd. Nawi, I. H. and Abd. Samad, Azman (2012) Successful plant regeneration of Orthosiphon stamineus from petiole. Journal of Medicinal Plants Research, 6 (26). pp. 4276-4280. ISSN 1996-0875

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Official URL: http://www.academicjournals.org/article/article138...


We present a successful regeneration of Orthosiphon stamineus plant from petiole. All of the explants were cultured on Murashige and Skoog (MS)-based medium supplemented with various concentrations of benzylaminopurine (BAP) and naphthalene acetic acid (NAA) at the growth condition (25 ± 2°C, 16-h photo period at light intensity of 22.85 μmol /m2 /s) for 8 weeks. Results showed that petiole explants cultured at combination of 1.0 mg/L BAP and 0.2 mg/L NAA gave highest number of shoot produced per explants (4.33 ± 0.33) and a maximum callus fresh weight gains (3.2 ± 1.9 g) as compared to other treatments. The first shoot was observed from petiole cultured at all treatments in 4 weeks culture except for 0.1 mg/L BAP + 0.5 mg/L NAA treatment. For root induction, treatment at 0.1 mg/L NAA produced the highest number of roots produced per shoot (9.8 ± 3.1) compared to other treatments. Regeneration comparison between petiole, young leaf and stem segments showed that petiole is the most suitable explant for an efficient plant regeneration system of O. stamineus.

Item Type:Article
Uncontrolled Keywords:Orthosiphon stamineus, plant regeneration, shoot regeneration
Subjects:Q Science
Divisions:Biosciences and Bioengineering
ID Code:33040
Deposited By: Fazli Masari
Deposited On:07 May 2014 01:53
Last Modified:05 Mar 2019 02:54

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