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Emergence of fungal pathogen Rhodotorula mucilaginosa and real-time PCR assay for future detection

Giek, Far Chan and Sinniah, Sivaranjini (2012) Emergence of fungal pathogen Rhodotorula mucilaginosa and real-time PCR assay for future detection. Scottish Journal of Arts, Social Sciences and Scientific Studies, 5 (1). pp. 90-100. ISSN 2047-1278

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Official URL: http://scottishjournal.co.uk/paper/SJASS_Vol.5_No....

Abstract

Rhodotorula species have shown significant worldwide prevalence emerged as fungal pathogen, mainly Rhodotorula mucilaginosa that causes fungemia. In this study, a molecular assay based on real-time PCR using EvaGreen was developed for sensitive and accurate detection of R. mucilaginosa. A pair of primers that specifically target this yeast was designed and the amplification assay was optimized using EvaGreen as the DNA binding dye. The assay could detect and quantify up to 100 fg concentration of genomic DNA from R. mucilaginosa with amplification efficiency of 101.8%. The quantitative real-time PCR assay merits future evaluation study in order to evaluate its potential as molecular diagnosis tool for early detection of rare fungal infection caused by R. mucilaginosa.

Item Type:Article
Uncontrolled Keywords:Mycosis, red yeast, Rhodotorula mucilaginosa, skin infection
Subjects:Q Science
Divisions:Biosciences and Bioengineering (Formerly known)
ID Code:31782
Deposited By: Fazli Masari
Deposited On:12 Jun 2013 12:59
Last Modified:25 Mar 2019 16:18

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