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Isolation and cloning of human NQO1 promoter in Pgl3 basic vector

Mohamed Zulkifli, Razauden and Chinigarzadeh, Asma and Yaze, Iman and Tajadod, Reyhaneh Rahnamai (2012) Isolation and cloning of human NQO1 promoter in Pgl3 basic vector. International Journal of Scientific & Technology Research , 1 (7). pp. 68-72. ISSN 2277-8616

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Abstract

Malaria is a major public health problem caused by Plasmodium falciparum, a parasite that infects red blood cells. Recently, several polyphenolic compounds have been reported capable of preventing the progression of malaria parasite. This observation may be related to NAD (P) H: quinon oxidoreductase: a flavoprotein responsible for catalyzing two-electron reduction and detoxification of quinones and their derivatives. In this study the 2123 bp of the 5` upstream of the first transcription start site was successfully isolated. Based on the bioinformatic program, several regulatory regions such as Antioxidant Response Element (ARE) may be responsible for the direct regulation of polyphenols on this enzyme. It was predicted at -477 from the first transcription start site. Upon isolation, this fragment was used in a cloning process into the pGL3 Basic vector and transformed to E.coli DH5a competent cells

Item Type:Article
Subjects:Q Science
Divisions:Biosciences and Bioengineering (Formerly known)
ID Code:30540
Deposited By: Liza Porijo
Deposited On:03 Mar 2014 09:24
Last Modified:13 Oct 2017 22:01

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