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The study of mammalian metabolism through NMR-based metabolomics

Salek, Reza and Cheng, Kian Kai and Griffin, Julian (2011) The study of mammalian metabolism through NMR-based metabolomics. Methods in Enzymology, 500 . pp. 337-351. ISSN 0076-6879

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Official URL: http://dx.doi.org/10.1016/B978-0-12-385118-5.00017...


High-resolution NMR spectroscopy has been widely used to monitor metabolism almost since the technique's development. It is now one of the principle technologies used in metabolomics, to profile the metabolite compliment of a cell, tissue, organism, or biofluid. This chapter describes how tissue extracts are prepared for NMR spectroscopy and, in particular, focuses on two approaches based on perchloric acid and methanol/chloroform extractions. This is followed by a description of key NMR experiments that can be used to profile tissue extracts, biofluids, or intact tissues. While these NMR techniques should be optimized for a particular sample set, we provide some tried and tested starting parameters for these experiments which should allow the user to acquire good quality spectra.

Item Type:Article
Uncontrolled Keywords:metabolome, tissue extraction, NMR spectroscopy, metabolic profiling, ¹H NMR
Subjects:Q Science > QL Zoology
Divisions:Chemical and Natural Resources Engineering
ID Code:29777
Deposited By: Fazli Masari
Deposited On:01 Apr 2013 04:57
Last Modified:23 Oct 2017 16:08

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