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Oleate induces apoptosis in 3T3-L1 adipocytes

A., Rohana and A. M., Fadzilah Adibah and M. S., Muhammad Roji (2011) Oleate induces apoptosis in 3T3-L1 adipocytes. International Journal of Biotechnology and Bioengineering, 5 (9). pp. 504-507. ISSN 2010-376X

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Oleic acid (C18:1) play an important role in proliferation of fat cells. In this study, the effect of oleate on cells viability in 3T3-L1 cells (fat cells) was investigated. The 3T3-L1 cells were treated with various concentrations of oleate in the presence of 23 mM glucose. Oleate was added to adipogenic media (day 0) to investigate the influence of oleate on proliferation of postconfluent preadipocytes after 24 h induction. 0.1 mM oleate promoted cell division by increasing 33.9% number of cells from basal control in postconfluent preadipocytes. However, there were no significantly different in cells viability with control cells when oleate concentrations were increased up to 0.5 mM. When added to differentiated adipocytes (day 12) for 48 h, the number of cells decreased as oleate concentrations increased. 92.7% of cells lost demonstrated apoptosis and necrosis after 48 h with 0.5 mM oleate. The fluorochrome staining was examined under fluorescence microscopy using acridine orange and ethidium bromide double staining. Furthermore, the presence of high lactate (60.6% increased from basal control) released into plasma has shown the direct cytotoxicity of 0.5 mM oleate on adipocytes.

Item Type:Article
Uncontrolled Keywords:adipocytes, apoptosis, oleate, postconfluent preadipocytes
Subjects:T Technology > TP Chemical technology
Divisions:Chemical and Natural Resources Engineering
ID Code:29666
Deposited By: Yanti Mohd Shah
Deposited On:21 Mar 2013 08:05
Last Modified:25 Apr 2019 01:18

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