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Protein recovery from surfactant precipitation

Shu, Ian Cheng and Stuckey, David C. (2011) Protein recovery from surfactant precipitation. Biotechnology Progress, 27 (6). pp. 1614-1622. ISSN 8756-7938

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Official URL: http://dx.doi.org/10.1002/btpr.671


The recovery of lysozyme from an aqueous solution containing precipitated lysozyme-AOT complexes formed by the direct addition of sodium bis-(2-ethylhexyl) sulfosuccinate (AOT) to a lysozyme solution was studied using both solvents, and a counterionic surfactant. Ethanol, methanol and solvent mixtures dissolved the surfactant precipitate and recovered lysozyme as a solid. Recovery efficiency and protein stability varied with the type of solvent used. An entirely different method of recovery was also evaluated using a counterionic surfactant: trioctylmethylammonium chloride (TOMAC) which bound to AOT releasing lysozyme into solution. Complete recovery (100%) of lysozyme was achieved at a molar ratio of 2:1 (TOMAC:AOT), and the original protein activity was maintained in the final aqueous phase. The recovered lysozyme retained its secondary structure as observed in circular dichroism (CD) spectra. Specific activity studies show that counterionic surfactant extraction does not alter the biological activity of the enzyme.

Item Type:Article
Uncontrolled Keywords:surfactant, precipitation, activity recovery, counterionic
Subjects:T Technology > TP Chemical technology
Divisions:Chemical and Natural Resources Engineering
ID Code:29382
Deposited By: Yanti Mohd Shah
Deposited On:13 Mar 2013 02:16
Last Modified:25 Mar 2019 08:07

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