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Identification of metabolites from phenanthrene oxidation by phenoloxidases and dioxygenases of polyporus sp. S133

Hadibarata, Tony and Tachibana, Sanro and Askari, Muhamad (2011) Identification of metabolites from phenanthrene oxidation by phenoloxidases and dioxygenases of polyporus sp. S133. Journal Of Microbiology And Biotechnology, 21 (3). pp. 299-304. ISSN 1017-7825

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Phenanthrene degradation by Polyporus sp. S133, a new phenanthrene-degrading strain, was investigated in this work. The analysis of degradation was performed by calculation of the remaining phenanthrene by gas chromatography - mass spectrometry. When cells were grown in phenanthrene culture after 92 h, all but 200 and 250 mg/l of the phenanthrene had been degraded. New metabolic pathways of phenanthrene and a better understanding of the phenoloxidases and dioxygenase mechanism involved in degradation of phenanthrene were explored in this research. The mechanism of degradation was determined through identification of the several metabolites; 9,10-phenanthrenequinone, 2,2'-diphenic acid, salicylic acid, and catechol. 9,10-Oxidation and ring cleavage to give 9,10-phenanthrenequinone is the major fate of phenanthrene in ligninolytic Polyporus sp. S133. The identification of 2,2'-diphenic acid in culture extracts indicates that phenanthrene was initially attacked through dioxigenation at C9 and C10 to give cis-9,10-dihydrodiol. Dehydrogenation of phenanthrene-cis-9,10-dihydrodiol to produce the corresponding diol, followed by ortho-cleavage of the oxygenated ring, produced 2,2'-diphenic acid. Several enzymes (manganese peroxidase, lignin peroxidase, laccase, 1,2-dioxygenase, and 2,3-dioxygenase) produced by Polyporus sp. S133 was detected during the incubation. The highest level of activity was shown at 92 h of culture.

Item Type:Article
Uncontrolled Keywords:dioxygenase, enzyme activity, metabolites, phenanthrene, phenoloxidase, polyporus sp. S133
Subjects:G Geography. Anthropology. Recreation > GE Environmental Sciences
Divisions:Water Research Alliance
ID Code:29138
Deposited By: Yanti Mohd Shah
Deposited On:21 Feb 2013 13:18
Last Modified:17 Mar 2019 03:02

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