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Identification of genes involved in the 4-aminobenzenesulfonate degradation pathway of hydrogenophaga sp PBC via transposon mutagenesis

Han, Ming Gan and Ibrahim, Zaharah and Shahir, Shafinaz and Yahya, Adibah (2011) Identification of genes involved in the 4-aminobenzenesulfonate degradation pathway of hydrogenophaga sp PBC via transposon mutagenesis. Fems Microbiology Letters, 318 (2). pp. 108-114. ISSN 0378-1097

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Official URL: http://dx.doi.org/10.1111/j.1574-6968.2011.02245.x

Abstract

Genes involved in the 4-aminobenzenesulfonate (4-ABS) degradation pathway of Hydrogenophaga sp. PBC were identified using transposon mutagenesis. The screening of 10000 mutants for incomplete 4-ABS biotransformation identified four mutants with single transposon insertion. Genes with insertions that impaired the ability to utilize 4-ABS for growth included (1) 4-sulfocatechol 1,2-dioxygenase ß-subunit (pcaH2) and 3-sulfomuconate cycloisomerase involved in the modified ß-ketoadipate pathway; (2) 4-aminobenzenesulfonate 3,4-dioxygenase component (sadA) involved in aromatic ring hydroxylation; and (3) transposase gene homolog with a putative cis-diol dehydrogenase gene located downstream. The pcaH2 mutant strain accumulated brown metabolite during growth on 4-ABS which was identified as 4-sulfocatechol through thin layer chromatography and HPLC analyses. Supplementation of wild-type sadA gene in trans restored the 4-ABS degradation ability of the sadA mutant, thus supporting the annotation of its disrupted gene.

Item Type:Article
Uncontrolled Keywords:4-aminobenzenesulfonate, Hydrogenophaga, electroporation, transposon insertion, Tn5
Subjects:Q Science
T Technology > TP Chemical technology
Divisions:Biosciences and Bioengineering (Formerly known)
ID Code:29132
Deposited By: Yanti Mohd Shah
Deposited On:21 Feb 2013 21:19
Last Modified:22 Oct 2020 12:04

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