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Emergence of aureobasidium pullulans as human fungal pathogen and molecular assay for future medical diagnosis

Chan, Giek Far and Puad, Mohamad Safwan Ahmad and Chin, Chai Fung and Abdul Rashid, Noor Aini (2011) Emergence of aureobasidium pullulans as human fungal pathogen and molecular assay for future medical diagnosis. Folia Microbiologica, 56 (5). pp. 459-467. ISSN 0015-5632

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Official URL: http://dx.doi.org/10.1007/s12223-011-0070-9


Despite the great importance of Aureobasidium pullulans in biotechnology, the fungus had emerged as an opportunistic human pathogen, especially among immunocompromised patients. Clinical detection of this rare human fungal pathogen presently relies on morphology diagnosis which may be misleading. Thus, a sensitive and accurate quantitative molecular assay for A. pullulans remains lacking. In this study, we presented the microscopy observations of A. pullulans that reveals the phenotypic plasticity of the fungus. A. pullulans-specific primers and molecular beacon probes were designed based on the fungal 18S ribosomal RNA (rRNA) gene. Comparison of two probes with varied quencher chemistry, namely BHQ-1 and Tamra, revealed high amplification efficiency of 104% and 108%, respectively. The optimized quantitative real-time PCR (qPCR) assays could detect and quantify up to 1 pg concentration of A. pullulans DNA. Both assays displayed satisfactory performance parameters at fast thermal cycling mode. The molecular assay has great potential as a molecular diagnosis tool for early detection of fungal infection caused by A. pullulans, which merits future study in clinical diagnosis.

Item Type:Article
Uncontrolled Keywords:invasive fungal infection, molecular beacon, invasive fungal disease, sabouraud dextrose agar, aureobasidium pullulans
Subjects:Q Science > QH Natural history > QH301 Biology
Divisions:Sustainability Research Alliance
ID Code:29036
Deposited By: Yanti Mohd Shah
Deposited On:21 Feb 2013 08:14
Last Modified:05 Mar 2019 01:37

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