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Analysis of a DNA computing readout method based on real-time PCR with taqman probes

Ibrahim, Zuwairie and Mohamed Saaid, Muhammad Faiz and Paramita, Adi Suryaputra and Suyama, Akira (2007) Analysis of a DNA computing readout method based on real-time PCR with taqman probes. In: International Conference On Control, Instrumentation & Mechatronics (CIM'07), 2007, Persada Johor International Convention Centre, Johor Bahru Johor.

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In the previous paper, a novel readout approach forthe Hamiltonian Path Problem (HPP) in DNAcomputing based on the real-time polymerase chainreaction (PCR) is proposed. Based on this approach,real-time amplification was performed with theTaqMan probes and the TaqMan detection mechanismwas exploited for the design and development of theproposed readout approach. The readout approachconsists of two steps: real-time amplification in vitrousing TaqMan-based real-time PCR, followed byinformation processing in silico to assess the results ofreal-time amplification, which in turn, enablesextraction of the Hamiltonian path. In this paper, theanalysis of the readout approach is presented, whichconsists of the comparison of the experimental resultwith that of conventional graduated PCR approach.Furthermore, the behavior of TaqMan-based real-timePCR is experimentally investigated in terms of theinitial concentration of template and TaqMan probes.

Item Type:Conference or Workshop Item (Paper)
Uncontrolled Keywords:Hamiltonian Path Problem (HPP), polymerase chainreaction (PCR), TaqMan-based
Subjects:T Technology > TK Electrical engineering. Electronics Nuclear engineering
Divisions:Electrical Engineering
ID Code:13765
Deposited By: Liza Porijo
Deposited On:12 Aug 2011 01:56
Last Modified:02 Aug 2017 06:53

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