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A Comparison of Leaf and Petal Senescence in Wallflower Reveals Common and Patterns of Gene Expression and Physiology

Price, Anna Marie and Orellana, Danilo F. and Stevens, Ryan and Acock, Rosemary and Faezah, Mohd Salleh and Wollaston, VickyBuchanan and Stead, Anthony D. and Rogers, Hilary J. (2008) A Comparison of Leaf and Petal Senescence in Wallflower Reveals Common and Patterns of Gene Expression and Physiology. Plant Physiology, 147 . pp. 1898-1912. ISSN 1532-2548 Online ; 0032-0889 Print

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Official URL: http://dx.doi.org/10.1104/pp.108.120402

Abstract

Petals and leaves share common evolutionary origins but perform very different functions. However, few studies have compared leaf and petal senescence within the same species. Wallflower (Erysimum linifolium), an ornamental species closely related to Arabidopsis (Arabidopsis thaliana), provide a good species in which to study these processes. Physiological parameters were used to define stages of development and senescence in leaves and petals and to align these stages in the two organs. Treatment with silver thiosulfate confirmed that petal senescence in wallflower is ethylene dependent, and treatment with exogenous cytokinin and 6-methyl purine, an inhibitor of cytokinin oxidase, suggests a role for cytokinins in this process. Subtractive libraries were created, enriched for wallflower genes whose expression is up-regulated during leaf or petal senescence, and used to create a microarray, together with 91 senescence-related Arabidopsis probes. Several microarray hybridization classes were observed demonstrating similarities and differences in gene expression profiles of these two organs. Putative functions were ascribed to 170 sequenced DNA fragments from the libraries. Notable similarities between leaf and petal senescence include a large proportion of remobilization-related genes, such as the cysteine protease gene SENESCENCEASSOCIATED GENE12 that was up-regulated in both tissues with age. Interesting differences included the up-regulation of chitinase and glutathione S-transferase genes in senescing petals while their expression remained constant or fell with age in leaves. Semiquantitative reverse transcription-polymerase chain reaction of selected genes from the suppression subtractive hybridization libraries revealed more complex patterns of expression compared with the array data

Item Type:Article
Uncontrolled Keywords:Petal Senescence,
Subjects:Q Science > QH Natural history > QH301 Biology
Divisions:Biosciences and Bioengineering (Formerly known)
ID Code:9886
Deposited By: Siti Najwa Hanim Kamarulzaman
Deposited On:25 Jun 2010 05:00
Last Modified:25 Jun 2010 05:01

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