The efficiency of DBD cold plasma pen treatment on the oyster mushroom bacterial decontamination

Abstract

Cold plasma provided bacterial inactivation role in food industry. In this study, the cold plasma play a crucial inactivation role when effectively reduces the bacteria colonies on oyster mushroom surface. By development of the dielectric barrier discharge-cold plasma pen (DBD-CPP) system, the mushroom surface was exposed to the cold plasma discharge with variable of exposure treatment time (0 min, 0.5 min, 1 min, 3 min and 5 min) with ~6 kV of power voltage and 5 SLM of atmospheric gas pressure flow rate. In order to identify the reduction of the microbial growth, isolation technique will be carry out by excising the mushroom sample into a suspension and serial dilution follows by identification of its colony morphologies and characteristics. Results screening shows increments of exposure treatment times up to 3 min shows none growth of bacteria colonies. This because the bacteria cell wall was disrupt and destruction by the plasma bombardment. Thus, this study able to extend the lifetime of the mushroom and produce a free microbial fresh mushroom by decontaminate the bacteria on the mushroom surface.