Variation in recombinant protein production volume of Fim-C-salmonella typhi as a raw material for typhoid detection kit at laboratory scale

Abstract

Salmonella typhi is gram-negative bacteria that caused typhoid fever in humans; prevention of the disease is currently through vaccination. The development of disease detection tools is also being carried out so that the detection process is faster and more accurate. In line with the development of typhoid detection devices, prior studies have managed to find factors that influence the production of Fim-C S. typhi protein on a small scale as raw material for typhoid detection kits. The purpose of this research is to apply the results of previous studies in the production of recombinant Fim-C-S. typhi proteins with volume variations of 50mL-300mL, as a foundation for large-scale production. The results of protein production were characterized by Sodium Dodecyl Sulfate Polyacrilamide Gel Electrophoresis (SDS-PAGE) and protein concentration measurements using the Bicinchoninic Acid (BCA) Assay at a wavelength of 562 nm. The results showed an amount of that protein increase along with gradually production volumes, mainly the protein in the form of inclusion bodies. According to the results obtained can be concluded production of the recombinant protein Fim-C-S. typhi at 50-300 mL volume variation on a laboratory scale has been successfully performed with consistent results, which is expected to be basic in production at pilot scale and large scale.