Initial study on the growth of haematococus pluvialis for astaxanthin production

Abstract

This research emphasizes the identification of best nutrients among the four medium that will be used for a better growth rate of Haematococcus pluvialis. The growth rate of H. pluvialis is controlled or regulated by the physical and chemical parameters. Each of the parameters gives different yield productions to H. pluvialis. Different types of medium composed of different nutrients composition resulted in various maturation and growth rate of H. pluvialis monitored by the change in the optical density at 750 nm over time. The best medium was Rudic`s medium which gave OD750nm at 0.757 after 20-day culture. Then, Bold Basal medium, Basal medium and BG11 medium were followed as 0.677 at day 20, 0.358 at day 12 and 0.162 at day 15, respectively. Besides, this research also focuses on identifying the most suitable carbon source. Sodium acetate was found to be the best in the cultivation of H. pluvialis with the maximum absorbance of 0.99 in Rudic`s medium. The higher cell density cultures was achieved with 4 g/L concentration of sodium acetate. Carbon source also promoted better maturation process of H. pluvialis which will enhance yield of astaxanthin production in a shorter period of time. The primary composition that contribute to the high growth of H. pluvialis can be determined by adding 50% of concentration CaCl2 in the medium with control flask contain 0.0585 g/L concentration of CaCl2. The growth of H. pluvialis at day 9 was shown to increase by 27%. with absorbance of 0.301 if compared to control which gave absorbance 0.236. High concentration of CaCl2 contribute to high growth of H. pluvialis.