Isolation and selection of fungi amylolytic and cellulolytic originated from Rhododendron sp. flower

Abstract

The purpose of this study was to isolate fungi (yeast and mold) originated from Rhododendron sp. flower from the Bali National Park. Fungi were isolated to the PDA medium based on the washing and direct method. The plates were kept for incubation at 28°C. The enzyme activity was assayed by blank disk and agar diffusion method. The positive results of amylase and cellulase enzymes were represented by the existence of spotless area on the selective medium after adding congo red and Lugol as a detecting agent. A total of 7 yeasts and 15 molds were isolated from Rhododendron sp. flower. The results of activity was revealed that 15 mold isolates positive for cellulase production (KP 2.1, 2.2, 2.3, 4.1, 4.2, 4.3, 8.1, 8.2, 8.3, 13.1, 13.2, 13.3, 16.1, 16.2, 16.3) 12 mold isolates positive for amylase production (KP 2.1, 2.2, 2.3, 8.1, 8.2, 8.3, 13.1, 13.2, 13.3, 16.1, 16.2, 16.3) and 7 yeast isolates positive for amylase and cellulase production (KH 1.2, 2.1, 2.2, 3.1, 3.2, 4.1, 4.2).