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Hypoglycemic and antidiabetic activities of Gynura procumbens extract

Mohd Bohari, Siti Pauliena and Hamid, Muhajir and Shaari, Khozirah and Lajis, Nordin and Begum, Rokeya and Ali, Liaquat and J.M, Abdul Hannan (2006) Hypoglycemic and antidiabetic activities of Gynura procumbens extract. In: 5th ANRAP International Seminar-MNPS 22nd Annual Seminar 2006, 8-10 November, 2006, Cititel, Midvalley , Kuala Lumpur.

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Abstract

The Malaysian herb,Gynura procumbens (also locally known as ‘sambung nyawa’) was extracted with methanol and partitioned into ethyl acetate, hexane and butanol fractions in order to investigate its hypoglycemic and antidiabetic activities. Methanolic extract of this plant showed considerably good effect on hypoglycemic Type1 and Type 2 diabetic rat models. Furthermore, the gut perfusion study using both crude methanolic extract and ethyl acetate fraction gave positive response, in which glucose absorption in the rat intestine was delayed. Insulin secretory study using rat pancreatic β-cell lines,BRIN- BD11, crude hexane and ethyl acetate fractions exhibited stimulatory response in insulin release. Glucose uptake assay using 3T3 mouse adipocytes cell lines indicated that the effect was dose dependent with crude hexane fraction giving highest stimulation to glucose uptake activity. However, the crude methanolic extract had the highest glucose uptake activity in L6 muscle cell lines. Initial analysis of the bioactive ethyl acetate fraction yielded mix of β- sitosterol and stigmasterol and kaempferol-3-O glucoside.

Item Type:Conference or Workshop Item (Speech)
Subjects:R Medicine > RM Therapeutics. Pharmacology
Divisions:?? DepBio ??
ID Code:931
Deposited By: Prof Dr. Noor Aini Abdul Rashid
Last Modified:01 Jun 2010 02:49

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