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The Effects of Plant Growth Regulators (PGR) on In Vitro Regeneration of Catharanthus roseus Linn.

UNSPECIFIED (2006) The Effects of Plant Growth Regulators (PGR) on In Vitro Regeneration of Catharanthus roseus Linn. In: Seminar on Medicinal and Aromatic Plants, 12 - 13 September 2006, FRIM, Malaysia. (Unpublished)

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Abstract

An efficient and reliable in vitro regeneration of the medicinal plant Catharanthus roseus (purple and white flowered) was developed. Different explants sources as well as plant growth regulators (PGR) with various concentrations were applied to determine the best source of explants and PGR for the in vitro regeneration of this plant. Even though both plants shared the same genus and species name, the results obtained from both plants differ. Generally, the best source of explants for both studies was the cotyledonary node with the highest survival rate among other explants source. However, the purple flowered plant produced the optimal shoot in the MS media supplemented with 1.0 mg/L BAP and 1.0 mg/L NAA with average 3.80  1.40 shoots per explants in contrast with the white flowered plant (0.2 mg/L BAP and 0.5 mg/L NAA with 3.67  2.49 average shoot number per explants). The best rooting capacity also differ for both plants where the purple flowered plant thrived in 1.5 mg/L IBA (85.7 %) while the white flower plant in 0.2 mg/L NAA (100%). Overall, the regeneration of C. roseus in vitro was achieved within 14 to 17 weeks.

Item Type:Conference or Workshop Item (Poster)
Subjects:Q Science > Q Science (General)
Divisions:Others
ID Code:9251
Deposited By: Ms. Zaidah Rahmat
Last Modified:17 Aug 2009 13:27

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