Universiti Teknologi Malaysia Institutional Repository

Plasmid Library Production from Local Medicinal Plant, Gardenia jasminoides

UNSPECIFIED (2007) Plasmid Library Production from Local Medicinal Plant, Gardenia jasminoides. In: The Regional Annual Fundamental Science Seminar, 28 - 29 May 2007, Johor, Malaysia. (Unpublished)

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Abstract

Attributing approximately 90% of the newly discovered pharmaceuticals, medicinal plants continue to be a powerful source of new drugs. One such local medicinal plant, Gardenia jasminoides, is gaining use in therapeutic aid for alleviating human ailments. Advancement in biotechnological methods of DNA-based study facilitates the determination of the suitable restriction enzyme for genomic DNA digestion and comparison of transformation method towards the plasmid library construction and subsequently, the screening procedure. Partial digestion of genomic DNA was achieved with HindIII restriction enzyme after 1 hour and 15 minutes digestion. Chemical transformation was a better method for library construction compared to Magic E.coli transformation method, giving higher transformation efficiency at 4 x 106 as compared to 8 x 104 for the latter method. PCR was used to evaluate the library in which 77% clone of various sizes were obtained from the library.

Item Type:Conference or Workshop Item (Speech)
Subjects:Q Science > Q Science (General)
Divisions:Others
ID Code:9248
Deposited By: Ms. Zaidah Rahmat
Last Modified:17 Aug 2009 10:29

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