Huyop, Fahrul Zaman and Hamzah, Ainon and Dayang Fredalina, Basri and Jaafar, Jafariah and Raja Abd Rahman, Raja Noor Zaliha (2008) Enzymatic study of the Dehalogenases involved in biodegradation of a herbicide 3-chloropropionic acid (3cp) by a novel indigenous bacteria. Project Report. Faculty of Biosciences and Bioengineering, Skudai, Johor. (Unpublished)
Full text not available from this repository.
Abstract
3-chloropropionic acid is synthetic halogenated compounds used in herbicide. A bacterium isolated from a soil sample and characterised as Rhodococcus sp. by 16S rRNA analysis, was able to degrade and utilised 3-chloropropionate as the sole source of carbon and energy. This was supported by the ability of the bacterium to grow on 20 mM 3-chloropropionate with a doubling time of 11.72 hours. The utilisation of 3-chloropropionate was also confirmed by detection of 3-chloropropionate depletion in the medium using HPLC (High Performance Liquid Chromatography). Cell free extract of Rhodococcus sp. had an enzyme specific activity of 0.013 μmol Cl-/min/mg protein towards 3-chloropropionate.
| Item Type: | Monograph (Project Report) |
|---|---|
| Uncontrolled Keywords: | 3-chloropropionic acid dehalogenase 3-chloropropionic acid |
| Divisions: | Science |
| ID Code: | 9065 |
| Deposited By: | Noor Aklima Harun |
| Deposited On: | 02 Jul 2009 01:26 |
| Last Modified: | 14 Aug 2017 06:45 |
Repository Staff Only: item control page
