Huyop, Fahrul Zaman and Hamzah, Ainon and Dayang Fredalina, Basri and Jaafar, Jafariah and Raja Abd Rahman, Raja Noor Zaliha (2008) Enzymatic Study Of The Dehalogenases Involved In Biodegradation Of A Herbicide 3-Chloropropionic Acid (3cp) By A Novel Indigenous Bacteria. Project Report. Faculty of Biosciences and Bioengineering, Skudai, Johor. (Unpublished)
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3-chloropropionic acid is synthetic halogenated compounds used in herbicide. A bacterium isolated from a soil sample and characterised as Rhodococcus sp. by 16S rRNA analysis, was able to degrade and utilised 3-chloropropionate as the sole source of carbon and energy. This was supported by the ability of the bacterium to grow on 20 mM 3-chloropropionate with a doubling time of 11.72 hours. The utilisation of 3-chloropropionate was also confirmed by detection of 3-chloropropionate depletion in the medium using HPLC (High Performance Liquid Chromatography). Cell free extract of Rhodococcus sp. had an enzyme specific activity of 0.013 Î¼mol Cl-/min/mg protein towards 3-chloropropionate.
|Item Type:||Monograph (Project Report)|
|Uncontrolled Keywords:||3-chloropropionic acid dehalogenase 3-chloropropionic acid|
|Deposited By:||Noor Aklima Harun|
|Deposited On:||02 Jul 2009 01:26|
|Last Modified:||02 Jun 2010 01:58|
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