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Enzymatic Study Of The Dehalogenases Involved In Biodegradation Of A Herbicide 3-Chloropropionic Acid (3cp) By A Novel Indigenous Bacteria

Huyop, Fahrul Zaman and Hamzah, Ainon and Dayang Fredalina, Basri and Jaafar, Jafariah and Raja Abd Rahman, Raja Noor Zaliha (2008) Enzymatic Study Of The Dehalogenases Involved In Biodegradation Of A Herbicide 3-Chloropropionic Acid (3cp) By A Novel Indigenous Bacteria. Project Report. Faculty of Biosciences and Bioengineering, Skudai, Johor. (Unpublished)

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Abstract

3-chloropropionic acid is synthetic halogenated compounds used in herbicide. A bacterium isolated from a soil sample and characterised as Rhodococcus sp. by 16S rRNA analysis, was able to degrade and utilised 3-chloropropionate as the sole source of carbon and energy. This was supported by the ability of the bacterium to grow on 20 mM 3-chloropropionate with a doubling time of 11.72 hours. The utilisation of 3-chloropropionate was also confirmed by detection of 3-chloropropionate depletion in the medium using HPLC (High Performance Liquid Chromatography). Cell free extract of Rhodococcus sp. had an enzyme specific activity of 0.013 μmol Cl-/min/mg protein towards 3-chloropropionate.

Item Type:Monograph (Project Report)
Uncontrolled Keywords:3-chloropropionic acid dehalogenase 3-chloropropionic acid
Divisions:Science
ID Code:9065
Deposited By: Noor Aklima Harun
Deposited On:02 Jul 2009 01:26
Last Modified:02 Jun 2010 01:58

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