Universiti Teknologi Malaysia Institutional Repository

Determination of Chromium(VI) reduction by acinetobacter haemolyticus using x-ray absorption fine structure spectroscopy

Hsiao, Pei Quek and Shahir, Shafinaz and Liu, Tao and Ahmad, Wan Azlina (2008) Determination of Chromium(VI) reduction by acinetobacter haemolyticus using x-ray absorption fine structure spectroscopy. Journal of Fundamental Sciences, 4 (2). pp. 415-422. ISSN 1823-626X

[img]
Preview
PDF - Published Version
281Kb

Official URL: http://jfs.ibnusina.utm.my/index.php/jfs/article/v...

Abstract

Several types of microorganisms have been reported to reduce Cr(VI) to the less toxic Cr(III) via enzymatic reactions. The main purpose of this study is to determine the reduction of Cr(VI) by a locally isolated bacterium, Acinetobacter haemolyticus (A. haemolyticus) using the x-ray absorption fine structure (XAFS) spectroscopy. XAFS analysis consisted of both the x-ray absorption near-edge structure (XANES) and extended x-ray absorption fine structure (EXAFS) spectra. XANES spectra of the rinsed Cr(VI)-laden A. haemolyticus biomass indicated that chromium was only present in the form of Cr (III). The nearest atoms coordinated to Cr(III) were oxygens in an octahedral geometry. The longer Cr-O bond lengths (~1.97 Å) obtained from the EXAFS spectra corroborated the presence of chromium as Cr(III) bonded to oxygen. These findings demonstrate the ability of A. haemolyticus to reduce Cr(VI) to Cr(III) that was bound to oxygen atoms of the ligands in A. haemolyticus

Item Type:Article
Uncontrolled Keywords:x-ray, EXAFS, XANES, chromium, reduction, Acinetobacter
Subjects:Q Science > QD Chemistry
Divisions:Science
ID Code:8974
Deposited By: Ms Zalinda Shuratman
Deposited On:01 Jul 2009 08:18
Last Modified:20 Oct 2010 08:43

Repository Staff Only: item control page