The effect of cinnamtannin B1 on cell proliferation and glucose uptake of 3T3-L1 cells

Abstract

The effects of cinnamtannin BI on cell proliferation and glucose uptake of 3T3-L1 cells were examined. Cinnamtannin BI promoted cell proliferation of 3T3-L1 adipocytes at a concentration range between 0. 11-0. 17 mM. The effect of cinnamtannin 131 on cellular 2-deoxy-D-[1-H-3] glucose uptake in differentiated 3T3-L1 adipocytes, following treatment with a 0.11 rnM concentration of cinnamtannin B1 for 15, 30 and 60 minutes, was an increase in the glucose uptake from a basal value to 702.0, 1111.0 and 2226.0 cpm, respectively (p<0.005). The comparable glucose uptakes with insulin treatment were 660.0, 1039.0 and 2135.0 cpm, respectively. Wortmannin and cytochalasin B were found to inhibit cinnamtannin B1-stimulated glucose uptake, but sodium orthovanadate increased the glucose uptake.