Rezayi, Majid and Ghayour-Mobarhan, Majid and Tavakoly Sany, Seyedeh Belin and Fani, Mona and Avan, Amir and Pasdar, Zahra and Ferns, Gordon A. and Abouzari-Lotf, Ebrahim and Amiri, Iraj S. (2018) A comparison of analytical methods for measuring concentrations of 25-hydroxy vitamin D in biological samples. Analytical Methods, 10 (47). pp. 5599-5612. ISSN 1759-9660
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Official URL: http://dx.doi.org/10.1039/c8ay02146e
Abstract
Serum 25-hydroxyvitamin D (25(OH)D) is the principal circulating metabolite of vitamin D. Several methods for measuring serum 25(OH)D have been reported. These methods include chromatographic methods (GC-MS, HPLC, and LC-MS), competitive protein-binding assay (CPBA), radioimmunoassay (RIA) and automated methods. In addition, biosensors have more recently been employed as potential candidates for routine analysis of 25(OH)D in serum. In this review, the common methods for quantification of 25(OH)D 2 and 25(OH)D 3 in biological samples, including the preparation of samples, are critically compared and discussed.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | protein binding, routine analysis, Vitamin-D |
| Subjects: | T Technology > TP Chemical technology |
| Divisions: | Chemical Engineering |
| ID Code: | 86751 |
| Deposited By: | Yanti Mohd Shah |
| Deposited On: | 30 Sep 2020 09:05 |
| Last Modified: | 30 Sep 2020 09:05 |
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