A portable sensor for cell optical density measurement in microfluidic chips

Abstract

This paper presents the development of a smartphone-controlled wireless device for cell optical density sensing in microfluidic chips. The footprint of the device is very compact relative to a classical laboratory spectrophotometer, making it a portable device. The cell optical density sensing device consists of an embedded microcontroller, optical sensing components, and a wireless transceiver performing cell optical density measurements in disposable microfluidic chips fabricated from poly(methylmethacrylate) polymers. The device is controlled by an Android application allowing for true portability and the possibility of remote or field operation of the device. The use of microfluidic chips as the sample carrier for optical density detection instead of a plastic cuvette allows users the flexibility to explore and/or conduct a variety of micro-scale chemical analysis using the device which would be difficult in a cuvette-based system. The function of the device is validated through a series of off-line and online optical density measurements using Saccharomyces cerevisae yeast cultures. The device is low cost, small enough to fit in most laboratory flow hood cabinets, and can be easily integrated into miniature bioreactor systems. Moreover, wireless communication enables the user to operate the device using smartphones or rapidly transfer the measured data to an online repository for analysis or storage.