Isolation of a New Efficient Dye Decolorizing White Rot Fungus Cerrena Sp WICC F39

Abstract

Lignin degrading system of white rot fungi and its exploitation in the decolorization of synthetic dyes has been used in a wide array for human benefits. In this study, decolorization of two model synthetic dyes, Reactive Black 5 (RB5) and Methylene Blue (MB) with the ability of Kraft lignin degradation were investigated using new fungal isolates from Malaysian soils. The most potent isolates WICC F38 and WICC F39 were selected for further investigation along with reference strain P. chrysosporium DSMZ 6909. To prove their dye decolorization capabilities, the qualitative and quantitative methods were conducted, while their potential for biodegradation of kraft lignin was carried out using gravimetric method. The degree of lignin degradation, dyes decolorization (dyes intensity reduction) and productivity of lignin peroxidase, manganese peroxidase, and laccase were studied. It was noticed that 86-88% of decolorization rate for both synthetic dyes was obtained by WICC F38 and WICC F39; respectively. However, MB showed a more decolorization rate than RB5 by the selected isolates. The efficiency of the selected fungal isolates comparisons with reference strain for decolorization rate of synthetic dyes could be ordered as follows; WICC F39> P. chrysosporium DSMZ 6909> WICC F38. The fungal decolorization capabilities in the present study were concomitant with the production of lignin peroxidase, manganese peroxidase and laccase. The isolates under the study were identified as Phanerochaete sp. WICC F38 (ac., KU141330) and Cerrena sp. WICC F39 (ac., KU141331) using molecular identification strategy.