Sanagi, Mohd Marsin and See, Hong Heng and Wan Ibrahim, Wan Aini and Abu Naim, Ahmedy (2006) Separation of tocol-derivatives by elevated temperature normal-phase liquid chromatography. Jurnal Teknologi C (45C). pp. 29-40. ISSN 0127-9696
Official URL: http://www.penerbit.utm.my/onlinejournal/45/C/JTDI...
A novel method for the separation of eight vitamin E isomers (ïƒ¡-, ïƒ¢-, ïƒ£-, ïƒ¤-tocopherol, and ïƒ¡-, ïƒ¢-, ïƒ£-, ïƒ¤ tocotrienol) and ïƒ¡-tocopherol acetate on normal phase high performance liquid chromatography (NP-HPLC) at elevated column temperature has been developed. The separation was optimized by varying the eluent composition and column temperature. All the isomers were successfully separated using NP-HPLC on amino and silica columns. By simply increasing the temperature for silica column, excellent separation efficiencies and shorter analysis times were achieved without significant loss in resolution. The developed separation method is rapid, shows excellent reproducibility, and suitable to be used as a quantitative method in analyzing tocopherols and tocotrienols.
|Uncontrolled Keywords:||Elevated temperature HPLC, tocopherols, tocotrienols, vitamin E|
|Subjects:||Q Science > QD Chemistry|
|Deposited By:||Pn Norhayati Abu Ruddin|
|Deposited On:||26 Feb 2009 08:51|
|Last Modified:||28 Oct 2010 04:56|
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