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Optimisation of growth medium for the production of cyclodextrin glucanotransferase from Bacillus stearothermophilus HR1 using response surface methodology

Abd. Rahman, Roshanida and Md. Illias, Rosli and Mohd Ghazali, Mohd Nawawi and Ismail, Ahmad Fauzi and Hassan, Osman and Kamaruddin, Kamarulzaman (2004) Optimisation of growth medium for the production of cyclodextrin glucanotransferase from Bacillus stearothermophilus HR1 using response surface methodology. Process Biochemistry, 39 . pp. 2053-2060. ISSN 0032-9592

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Official URL: http://dx.doi.org/10.1016/j.procbio.2003.10.014

Abstract

Cyclodextrin glucanotransferase (CGTase) activity was observed when the bacterium was grown in the medium at various initial pH values, containing carbon, nitrogen, phosphorus and mineral salt sources at 50 oC for 24 h in the shake flasks. The optimisation of this growth medium was carried out using response surface methodology. The design contains a total of 32 experimental trials involving 10 star points and 6 replicates at the centre points. The design was employed by selecting sago starch, peptone from casein, K2HPO4, CaCl2 and initial pH as five independent variables in this study. The optimal calculated values of tested variables for maximal production of CGTase were found to be comprised of: sago starch, 16.02 g/l; peptone from casein, 20 g/l; K2HPO4, 1.4 g/l; CaCl2, 0.2 g/l and initial pH, 7.54 with a predicted CGTase activity of 14.20 U/ml. These predicted optimal parameters were tested in the laboratory and the final CGTase activity obtained was very close to the predicted value at 14.80 U/ml.

Item Type:Article
Uncontrolled Keywords:Cyclodextrin glucanotransferase, experimental design
Subjects:T Technology > TP Chemical technology
Divisions:Chemical and Natural Resources Engineering (Formerly known)
ID Code:772
Deposited By: Pn Norazana Ibrahim
Deposited On:11 Mar 2008 10:09
Last Modified:01 Jun 2010 02:47

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